Journal: Biomedicines

Article Title: An Overview of Methods for Cardiac Rhythm Detection in Zebrafish

doi: 10.3390/biomedicines8090329

Figure Lengend Snippet: Comparison of the methods for cardiac rhythm detection in zebrafish embryos.

Article Snippet: (Gierten, Pylatiuk et al., 2019) , Yes , Yes , MATLAB (dynamic pixel changes method) , An ACQUIFER wide-field high content screening microscope equipped with a white LED array , Heart , Whole heartbeat frequency , Yes , [ ] .

Techniques: Comparison, Transgenic Assay, Software, Inverted Microscopy, Microscopy, High Content Screening, Light Microscopy

Journal: PLoS ONE

Article Title: Glucose Controls Morphodynamics of LPS-Stimulated Macrophages

doi: 10.1371/journal.pone.0096786

Figure Lengend Snippet: RAW 264.7 macrophages expressing Lifeact-EYFP were pre-incubated in control medium or medium containing 2.5 µM oligomycin and 25 mM glucose (A), 10 mM 2-DG and 25 mM glucose (B), 10 mM galactose and no glucose (C), or 1 mM glucose and 10 mM galactosel (D) medium for the indicated time periods. To assess spreading efficiency, cells were detached with EDTA, re-suspended, seeded in 96 well plates and allowed to adhere. Cell spreading of EYFP-positive cells was recorded over time using a BD Pathway high content microscope. The average pixel area per cell was determined at 10 minute intervals. Lines and bars represent means ± SEM of three independent experiments performed in triplicate. For every condition, representative images of cells at 0 and 200 minutes are presented in the panel on the right.

Article Snippet: The plate was immediately imaged for 24 hours, continuously, on a BD Pathway high-content spinning disc confocal microscope equipped with a temperature and CO 2 controllable incubation chamber, using a 20x objective and 2×2 montage capture.

Techniques: Expressing, Incubation, Microscopy